Journal ArticleAuthors : Fasano, Andrea (2023)
The biodiversity of hydrogenases, the enzymes that oxidize and produce H2, is only just beginning to
be explored. Here we use direct electrochemistry to characterize two enzymes from a subgroup of
ancestral FeFe hydrogenases, defined by the presence of three adjacent cysteine residues near the
active site: the third FeFe hydrogenase from Clostridium pasteurianum (CpIII) and the second from
Megasphaera elsdenii (MeII). To examine the functio...