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Browsing by Author Nakahara, Emi

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  • OER000002683.pdf.jpg
  • Journal article

  • Development of a new DHFR-based destabilizing domain with enhanced basal turnover and applicability in mammalian systems

    • Authors : Nakahara, Emi (2023)

  • Destabilizing domains (DDs) are an attractive strategy allowing for positive post-transcriptional small molecule-regulatable control of a fusion protein’s abundance. Yet in many instances, the currently available DDs suffer from higher-than-desirable basal levels of the fusion protein. Accordingly, we redesigned the E. coli dihydrofolate reductase (ecDHFR) DD by introducing a library of ~1200 random ecDHFR mutants fused to YFP into CHO cells. Following su...