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Browsing by Author Udema, Ikechukwu Iloh

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Journal article

Direct Estimate of the Specificity Constant: A Possibility or a Fluke? Pre-steady-state Substrate Concentrations and Enabling Mathematical Equations

Authors : Udema, Ikechukwu Iloh (2023)

A high-ranking scientist has recently proposed the need for direct estimation of the specificity constant rather than by calculation after a separate determination of maximum velocity and the Michaelis-Menten constant (KM). The objectives of this study are to derive novel equations for the zero-arbitrary determination of pre-steady-state (PSS) concentration of the substrate suitable for PSS assays, direct estimation of specificity constant (SC) under ...

Journal article

Where Initial rates are directly proportional to Substrate concentrations with Application in Molar-mass Determination, Zero-order Specificity constant is Inappropriate

Authors : Udema, Ikechukwu Iloh (2023)

High-ranking scientists" employ the initial rate (vi), expression without consideration for 30 the conditions under which the vi expression can be used. The consequence is the suggestion that the vi 31 is equal to the product of maximum velocity, Vmax, and substrate concentration [S0] divided by the 32 Michaelis-Menten constant, KM. The main objectives are: 1) to show that vi is not equal to Vmax[S0]/KM; 2) to show that&#...