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Browsing by Author Yanagisawa, Haruaki

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  • OER000000737.pdf.jpg
  • Journal article

  • Cryo-electron tomography of cardiac myofibrils reveals a contraction-induced lattice twist in the Z-discs

    • Authors : Oda, Toshiyuki; Yanagisawa, Haruaki (2020)

  • The Z-disc forms a boundary between sarcomeres, which constitute structural and functional units of striated muscle tissue. Actin filaments from adjacent sarcomeres are cross-bridged by α-actinin in the Z-disc, allowing transmission of tension across the myofibril. Despite decades of studies, the 3D structure of Z-disc has been elusive due to the limited resolution of conventional electron microscopy. Here, we observed porcine cardiac myofibrils using cryo-electron...
  • OER000000920.pdf.jpg
  • Journal article

  • Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin

    • Authors : Oda, Toshiyuki; Yanagisawa, Haruaki; Wakabayashi, Takeyuki (2020)

  • Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct&#...