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Browsing by Subject G-quadruplex

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Showing results 1 to 6 of 6

Journal article

A mechanism for the extension and unfolding of parallel telomeric G-quadruplexes by human telomerase at single-molecule resolution

Authors : Paudel, Bishnu P; Moye, Aaron Lavel; Assi, Hala Abou (2020)

Telomeric G-quadruplexes (G4) were long believed to form a protective structure at telomeres, preventing their extension by the ribonucleoprotein telomerase. Contrary to this belief, we have previously demonstrated that parallel-stranded conformations of telomeric G4 can be extended by human and ciliate telomerase. However, a mechanistic understanding of the interaction of telomerase with structured DNA remained elusive. Here, we use single-molecule fluorescence resonance energy...

Journal article

Decoding mRNA translatability and stability from 5’UTR

Authors : Jia, Longfei; Mao, Yuanhui; Ji, Quanquan (2020)

Precise control of protein synthesis by engineering sequence elements in 5’ untranslated region (5’UTR) remains a fundamental challenge. To accelerate our understanding of cis-regulatory code embedded in 5’UTR, we devised massively parallel reporter assays from a synthetic mRNA library composed of over one million 5’UTR variants. A completely randomized 10-nucleotide sequence preceding an upstream open reading frame (uORF) and downstream GFP leads to a broad range...

Journal article

Detect Genomic G-Quadruplexes in Living Animal Cells with a Tiny Artificial Protein Probe

Authors : Zheng, Ke-wei; Zhang, Jia-yu; He, Yi-de (2020)

G-quadruplex (G4) structures formed by guanine-rich nucleic acids are implicated in essential physiological processes and serve as important drug targets. The genome-wide detection of G4s in living cells is important for exploring the biological role of G4s but has not yet been achieved due to the lack of a suitable G4 probe. We engineered a 6.7 kDa G4 probe (G4P) protein that binds G4s with high affinity and specificity. We used it&#...

Journal Article

Discovery of novel G-quadruplex stabilizing compounds from medicinal plant & Evaluates their cellular toxicity

Authors : Ranjan, Prashant (2023)

G-Quadruplexes (G4Q) are higher-order, four-stranded structures that can be formed by repeated guanine tracts in human genomes. In this study, we used a structure-based virtual screening of phytomolecules derived from medicinal plants in order to discover new natural DNA G4Q binders. The top 40 ligands were sorted out based on binding affinity values after completing a docking study on 314 phytomolecule samples with parallel and mixed ...

Journal article

The C9Orf72 hexanucleotide repeat expansion aggregates by means of multimolecular G-quadruplex formation

Authors : Raguseo, Federica (2023)

Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are two neurodegenerative diseases genetically characterised by the expansion of the hexanucleotide repeat (GGGGCC)n. Here we investigated the formation of nucleic-acid secondary structures in the expansion repeats, and their role in generating aggregates that are characteristic of ALS/FDT. We observed significant aggregation of DNA (GGGGCC)n, which we ascribe to the formation of multimolecular G-quadruplexes&...

Journal Article

The VEGF G-quadruplex forming promoter is repaired via long-patch BER

Authors : Hussen, Adil (2023)

In response to oxidative damage, base excision repair (BER) enzymes perturb the structural equilibrium of the VEGF promoter between B-form and G4 DNA conformations, resulting in epigenetic-like modifications of gene expression. However, the mechanistic details remain enigmatic, including the activity and coordination of BER enzymes on the damaged G4 promoter. To address this, we investigated the ability of each BER factor to conduct its repair&#...