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  • Journal article

  • Comparison of protein and peptide fractionation approaches in protein identification and quantification from Saccharomyces cerevisiae

    • Tác giả : Deng, Liting; Handler, David C. L; Multari, Dylan (2020)

  • Proteomics, as a high-throughput technology, has been developed with the aim of investigating the maximum number of proteins in cells. However, protein discovery and data generation vary in depth and coverage when different technical strategies are used. In this study, four different sample preparation, and peptide or protein fractionation, methods were applied to identify and quantify proteins from log-phase yeast lysate: sodium dodecyl sulfate-polyacrylamide gel ...