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Browsing by Author Frei, Michelle S.

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  • OER000002972.pdf.jpg
  • Journal article

  • HaloTag9: an engineered protein tag to improve fluorophore performance

    • Authors : Frei, Michelle S. (2021)

  • HaloTag9 is an engineered variant of HaloTag7 with up to 40% higher brightness and increased fluorescence lifetime when labeled with fluorogenic rhodamines. Moreover, combining HaloTag9 with HaloTag7 and other fluorescent probes enabled live-cell multiplexing using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. The increased brightness of HaloTag9 and its use in fluorescence lifetime multiplexing makes it a...