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Browsing by Author Friedman, Larry J

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  • OER000000399.pdf.jpg
  • Journal article

  • Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription

    • Authors : Rosen, Grace A; Baek, Inwha; Friedman, Larry J (2020)

  • The synthesis of a eukaryotic messenger RNA molecule involves the association of RNA polymerase and dozens of accessory proteins on DNA. We used differently colored fluorescent dyes to tag DNA, RNA polymerase II, and the elongation factor Spt4/5 in yeast nuclear extract, and then observed the assembly and dynamics of individual molecules of the proteins with single DNA molecules by microscopy. The observations quantitatively define an overall pathwa...
  • OER000000871.pdf.jpg
  • Industry article

  • Mechanism of upstream promoter element stimulation of transcription at a ribosomal RNA promoter determined by single-molecule imaging

    • Authors : Mumm, Jeffrey P; Friedman, Larry J; Gelles, Jeff (2020)

  • DNA elements upstream of transcription promoters play a role in regulating transcription initiation in all organisms. In bacteria, upstream A-T rich sequences called UP elements can stimulate transcription through contact with the α subunit C-terminal domain (αCTD) of core RNA polymerase (RNAP), but the kinetic mechanisms by which they do so remain unclear. We investigated the role of the UP element in stimulating initiation from the strong E. coli&...