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Browsing by Author Goldman, Daniel H

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Journal article

Dynamic regulation of translation quality control associated with ribosome stalling

Authors : Goldman, Daniel H; Livingston, Nathan M; Movsik, Jonathan (2020)

Translation of problematic mRNA sequences induces ribosome stalling. Collided ribosomes at the stall site are recognized by cellular quality control machinery, resulting in dissociation of the ribosome from the mRNA and subsequent degradation of the nascent polypeptide and in some organisms, decay of the mRNA. However, the timing and regulation of these processes are unclear. We developed a SunTag-based reporter to monitor translation in real time on sin...

Periodicals (Báo – Tạp chí)

Puromycin reactivity does not accurately localize translation at the subcellular level

Authors : Enam, Syed Usman; Zinshteyn, Boris; Goldman, Daniel H (2020)

Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor. The development of puromycin antibodies and derivatized puromycin analogs has enabled the quantification of active translation in bulk and single-cell assays. More recently, in vivo puromycylation assays have become popular tools for loc...