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Browsing by Author Hurley, James H

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  • OER000000801.pdf.jpg
  • Journal article

  • A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy

    • Authors : Fracchiolla, Dorotea; Chang, Chunmei; Hurley, James H (2020)

  • Autophagy requires the synthesis of PI(3)P and the conjugation of LC3 to the phagophore membrane. We reconstituted these two reactions and their coupling by WIPI2, and showed that positive feedback between PI3KC3-C1 and WIPI2 leads to rapid LC3 lipidation by the ATG16L1 complex.
  • OER000000739.pdf.jpg
  • Journal article

  • Structure of the lysosomal SCARF (L-SCARF) complex, an Arf GAP haploinsufficient in ALS and FTD

    • Authors : Yuan Su, Ming; Zoncu, Roberto; Hurley, James H (2020)

  • Mutation of C9ORF72 is the most prevalent defect in amyotrophic lateral sclerosis (ALS) and frontal temporal degeneration (FTD). Together with hexanucleotide repeat expansion, haploinsufficiency of C9ORF72 contributes to neuronal dysfunction. We determined the structure of the SMCR8-C9orf72-WDR41 complex by cryo-EM. C9orf72 and SMCR8 are both longin-DENN domain proteins, while WDR41 is a beta-propeller protein that binds to SMCR8 such that the whole structure resembles ...