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Browsing by Author Martynowycz, Michael W

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  • OER000000415.pdf.jpg
  • Journal article

  • Efficient, high-throughput ligand incorporation into protein microcrystals by on-grid soaking

    • Authors : Martynowycz, Michael W; Gonen, Tamir (2020)

  • A method for soaking ligands into protein microcrystals on TEM grids is presented. Every crystal on the grid is soaked simultaneously using only standard cryoEM vitrification equipment. The method is demonstrated using proteinase K microcrystals soaked with the 5-amino-2,4,6-triodoisophthalic acid (I3C) magic triangle. A soaked microcrystal is milled to a thickness of 200nm using a focused ion-beam, and microcrystal electron diffraction (MicroED) data are collected.&#x...