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Browsing by Author Takada, Hiraku

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  • OER000000932.pdf.jpg
  • Industry article

  • Ribosome association primes the stringent factor Rel for recruitment of deacylated tRNA to ribosomal A-site

    • Authors : Takada, Hiraku; Roghanian, Mohammad; Montes, Julien Caballero (2020)

  • In the Gram-positive Firmicute bacterium Bacillus subtilis, amino acid starvation induces synthesis of the alarmone (p)ppGpp by the multi-domain RelA/SpoT Homolog factor Rel. This bifunctional enzyme is capable of both synthesizing and hydrolysing (p)ppGpp. To detect amino acid deficiency, Rel monitors the aminoacylation status of the ribosomal A-site tRNA by directly inspecting the tRNA’s CCA end. Here we uncover the molecular mechanism of Rel-mediated stringent&...
  • OER000000918.pdf.jpg
  • Journal article

  • The C-terminal RRM/ACT domain is crucial for fine-tuning the activation of ‘long’ RelA-SpoT Homolog enzymes by ribosomal complexes

    • Authors : Takada, Hiraku; Roghanian, Mohammad; Murina, Victoriia (2020)

  • The (p)ppGpp-mediated stringent response is a bacterial stress response implicated in virulence and antibiotic tolerance. Both synthesis and degradation of the (p)ppGpp alarmone nucleotide are mediated by RelA-SpoT Homolog (RSH) enzymes which can be broadly divided in two classes: single-domain ‘short’ and multi-domain ‘long’ RSH. The regulatory ACT (Aspartokinase, Chorismate mutase and TyrA) / RRM (RNA Recognition Motif) domain is a near-universal C-terminal domain&#x...