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Browsing by Author Warnasooriya, Chandani

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  • OER000004005.pdf.jpg
  • Journal article

  • A Splice Site-Sensing Conformational Switch in U2AF2 is Modulated by U2AF1 and its Recurrent Myelodysplasia-Associated Mutation

    • Authors : Warnasooriya, Chandani; Glasser, Eliezra; Feeney, Callen F (2019)

  • An essential heterodimer of the U2AF1 and U2AF2 pre-mRNA splicing factors nucleates spliceosome assembly at polypyrimidine (Py) signals preceding the major class of 3ʹ splice sites. Among myelodysplastic syndromes (MDS), U2AF1 frequently acquires an S34F-encoding mutation. The influence of the U2AF1 subunit and its S34F mutation on the U2AF2 conformations remains unknown. Here, we employ single molecule Förster resonance energy transfer (FRET) to determine the inf...
  • OER000000732.pdf.jpg
  • Journal article

  • Extending the spacing between the Shine-Dalgarno sequence and P-site codon reduces the rate of mRNA translocation

    • Authors : Wakabayashi, Hironao; Warnasooriya, Chandani; Ermolenko, Dmitri N (2020)

  • By forming basepairing interactions with the 3’ end of 16S rRNA, mRNA Shine-Dalgarno (SD) sequences positioned upstream of Open Reading Frames (ORFs) facilitate translation initiation. During the elongation phase of protein synthesis, intragenic SD-like sequences stimulate ribosome frameshifting and may also slow down ribosome movement along mRNA. Here, we show that the length of the spacer between the SD sequence and P-site codon strongly affects the rate&#x...