Duyệt theo

Hồ sơ tác giả

Tìm kiếm theo: Chủ đề ái lực

Duyệt theo: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
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  • OER000002539.pdf.jpg
  • Journal article

  • Affinity-ranking BIN1 interactions underpinning centronuclear myopathy

    • Tác giả : Zambo, Boglarka  (2023)

  • Deletion of the protein-protein interaction SH3 domain of the membrane remodeling amphiphysin 2 (BIN1) protein was found to lead to centronuclear myopathy in patients, yet only few interaction partners of BIN1 SH3 have been identified so far, precluding a better understanding of the pathomechanism. Here we used the holdup assay to proteome-wide measure steady-state affinity constants of BIN1 SH3 domain for thousands of full-length cellular...
  • OER000002626.pdf.jpg
  • Journal article

  • Ultrahigh-affinity transport proteins from ubiquitous marine bacteria reveal mechanisms and global patterns of nutrient uptake

    • Tác giả : Clifton, Ben E. (2023)

  • SAR11 bacteria are the most abundant members of the global ocean microbiome and have a broad impact on ocean ecosystems. To thrive in their competitive oligotrophic environments, these bacteria rely on solute-binding proteins (SBPs) that facilitate nutrient uptake through ABC transporters. Nonetheless, previous studies have been unable to access the molecular mechanisms and functions of these transporters because they rely heavily on homology-based p...
  • OER000002648.pdf.jpg
  • Journal article

  • Universal Design Principle to Enhance Enzymatic Activity using the Substrate Affinity

    • Tác giả : Ooka, Hideshi (2023)

  • Design principles to improve enzymatic activity are essential to promote energy-material conversion using biological systems. For more than a century, the Michaelis-Menten equation has provided a fundamental framework of enzymatic activity. However, there is still no concrete guideline on how the parameters should be optimized to enhance enzymatic activity. Here, we demonstrate that tuning the Michaelis-Menten constant (𝐾𝑚) to the substrate concentration (𝑆) maximizes&#x...