Browse

Author Profile

Browsing by Subject Định lượng

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:  
Showing results 1 to 2 of 2
  • OER000002480.pdf.jpg
  • Journal Article

  • Label-free Quantification of Host-Cell Protein Impurity in a Recombinant Hemoglobin Reference Material

    • Authors : Henrion, André (2023)

  • Quantitative analysis depends on pure-substance primary calibrators with known mass fractions of impurity. Here, label-free quantification (LFQ) is being evaluated as a readily available, reliable method for determining the mass fraction of host-cell proteins (HCPs) in bioengineered proteins. For example, hemoglobin-A2 (HbA2) is being used as obtained through overexpression in E.coli. Two different materials had been produced: natural, and U-15N-labeled HbA2. For quantification&...
  • OER000002669.pdf.jpg
  • Journal article

  • Nucleic acid quantification with amplicon yield in recombinase polymerase amplification

    • Authors : Roy, Rahul (2023)

  • Amplification-based qPCR provides accurate and sensitive nucleic acid quantification. However, the requirement of temperature cycling and real-time monitoring limits its translation to different settings. Here, we adapted isothermal Recombinase Polymerase Amplification (RPA) reaction to develop a semi-quantitative method that relies on final amplicon yield to estimate initial target nucleic acid copy number. To achieve this, we developed a phenomenological model&#...