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Browsing by Subject Động học enzyme

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Journal article

A two-point IC50 method for evaluating the biochemical potency of irreversible enzyme inhibitors

Authors : Kuzmič, Petr (2023)

Irreversible (covalent) enzyme inhibitors cannot be unambiguously ranked for biochemical potency by using IC50 values determined at a single point in time, because the same IC50 value could originate either from relatively low initial binding affinity accompanied by high chemical reactivity, or the other way around. To disambiguate the potency ranking of covalent inhibitors, here we describe a data-analytic procedure relying on two separate IC50...

Journal article

Diverse Phosphoserine Phosphatases Exhibit Maximum Activity at an Intermediate Binding Affinity in Accord with the Sabatier Principle of Catalysis

Authors : Chiba, Yoko (2023)

A unified framework to rationalize enzymatic activity is essential to understand cellular function and metabolic evolution. Recent studies have shown that the activity of several hydrolases is maximized when the substrate binding affinity (Michaelis-Menten constant:) is neither too strong nor too weak. This is because an intermediate resolves the trade-off between, in accord with the Sabatier principle of artificial catalysis. However, it remains unc...