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  • OER000002940.pdf.jpg
  • Journal article

  • Cell-free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs

    • Authors : Do, Hoa Quynh (2021)

  • The Proton-Coupled Folate Transporter (PCFT) is a transmembrane transport protein that controls the absorption of dietary folates in the small intestine. PCFT also mediates uptake of chemotherapeutically used antifolates into tumor cells. PCFT has been identified within lipid rafts observed in phospholipid bilayers of plasma membranes, a micro environment that is altered in tumor cells. The present study aimed at investigating the ...
  • OER000002472.pdf.jpg
  • Journal Article

  • Factors influencing the detergent-free membrane protein isolation using synthetic nanodisc-forming polymers

    • Authors : Krishnarjuna, Bankala (2023)

  • The detergent-free isolation of membrane proteins using synthetic polymers is becoming the desired approach for functional and structural studies of membrane proteins. Since the expression levels for many membrane proteins are low and a high yield of functionalized reconstituted membrane proteins is essential for in vitro studies, it is crucial to optimize the experimental conditions for a given polymer to effectively solubilize target membranes/...