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Showing results 1 to 8 of 8
  • OER000002975.pdf.jpg
  • Journal article

  • A biosensor to gauge protein homeostasis resilience differences in the nucleus compared to cytosol of mammalian cells

    • Authors : Raeburn, Candice B. (2021)

  • An extensive network of chaperones and other proteins maintain protein homeostasis and guard against inappropriate protein aggregation that is a hallmark of neurodegenerative diseases. Using a fluorescence resonance energy-based biosensor that simultaneously reports on intact cellular chaperone holdase activity and detrimental aggregation propensity, we investigated the buffering capacity of the systems managing protein homeostasis in the nucleus of the human cell l...
  • OER000002922.pdf.jpg
  • Journal article

  • Biochemical insight into novel Rab-GEF activity of the mammalian TRAPPIII complex

    • Authors : Harris, Noah J (2021)

  • Transport Protein Particle complexes (TRAPP) are evolutionarily conserved regulators of membrane trafficking, with this mediated by their guanine nucleotide exchange factor (GEF) activity towards Rab GTPases. In metazoans evidence suggests that two different TRAPP complexes exist, TRAPPII and TRAPPIII. These two complexes share a common core of subunits, with complex specific subunits (TRAPPC9 and TRAPPC10 in TRAPPII and TRAPPC8, TRAPPC11, TRAPPC12, TRAP...
  • OER000002920.pdf.jpg
  • Journal article

  • Discovery of a pre-mRNA structural scaffold as a contributor to the mammalian splicing code

    • Authors : Saha, Kaushik (2021)

  • The specific recognition of splice signals at or near exon-intron junctions is not explained by their weak conservation and instead is postulated to require a multitude of features embedded in the pre-mRNA strand. We explored the possibility of three-dimensional structural scaffold of AdML – a model pre-mRNA substrate – guiding early spliceosomal components to the splice signal sequences. We find that mutations in the non...
  • OER000002947.pdf.jpg
  • Journal article

  • Flexibility of a mammalian lipidome — insights from mouse lipidomics

    • Authors : Surma, Michał A. (2023)

  • Lipidomics has become an indispensable method for the quantitative assessment of lipid metabolism in basic, clinical, and pharmaceutical research. It allows for the generation of information-dense datasets in a large variety of experimental setups and model organisms. Previous studies, mostly conducted in mice (Mus musculus), have shown a remarkable specificity of the lipid compositions of different cell types, tissues, and organs. However, a systema...
  • OER000002344.pdf.jpg
  • Journal Article

  • nfluence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles

    • Authors : Gookin, Timothy E. (2023)

  • Proteasomes are multi-subunit, multi-catalytic protein complexes present in eukaryotic cells that degrade misfolded, damaged, or unstructured proteins. In this study, we used an activity-guided proteomic methodology based on a fluorogenic peptide substrate to characterize the composition of proteasome complexes in WT yeast, and the changes these complexes undergo upon the deletion of Pre9 (Δα3) or of Sem1 (ΔSem1). A comparison of whole-cell proteomi...
  • OER000002423.pdf.jpg
  • Ebooks (Sách điện tử)

  • Stable mammalian serum albumins designed for bacterial expression

    • Authors : Khersonsky, Olga (2023)

  • Albumin is the most abundant protein in the blood serum of mammals and has essential carrier and physiological roles. Albumins are also used in a wide variety of molecular and cellular experiments and in the cultivated meat industry. Despite their importance, however, albumins are challenging for heterologous expression in microbial hosts, likely due to 17 conserved intramolecular disulfide bonds. Therefore, albumins used in research and bi...
  • OER000002371.pdf.jpg
  • Journal Article

  • Structural characterization of ligand binding and pH-specific enzymatic activity of mouse Acidic Mammalian Chitinase

    • Authors : Díaz, Roberto Efraín (2023)

  • Chitin is an abundant biopolymer and pathogen-associated molecular pattern that stimulates a host innate immune response. Mammals express chitin-binding and chitin-degrading proteins to remove chitin from the body. One of these proteins, Acidic Mammalian Chitinase (AMCase), is an enzyme known for its ability to function under acidic conditions in the stomach but is also active in tissues with more neutral pHs, such as the lung. Here, w...
  • OER000003043.pdf.jpg
  • Journal article

  • The Mammalian Cytosolic Type 2 (R)-β-hydroxybutyrate Dehydrogenase (BDH2) is 4-oxo-L-proline Reductase (EC 1.1.1.104)

    • Authors : Kwiatkowski, Sebastian P. (2021)

  • The early studies on chicken embryos revealed that exposition to 4-oxo-L-proline resulted in the explicit increase in 4-hydroxy-L-proline content in their tissues. In 1962, 4-oxo-L-proline reductase, an enzyme responsible for the reduction of 4-oxo-Lproline, was partially purified from rabbit kidneys and characterized biochemically, but only recently the molecular identity of the enzyme has been unveiled in our laboratory. The present investigation reports the purific...