Journal ArticleAuthors : Ishihama, Yasushi (2023)
Three representative protein kinases with different substrate preferences, ERK1 (Prodirected),
CK2 (acidophilic), and PKA (basophilic), were used to investigate phosphorylation
sequence motifs in substrate pools consisting of the proteomes from three different cell lines,
MCF7 (human mammary carcinoma), HeLa (human cervical carcinoma), and Jurkat (human
acute T-cell leukemia). Specifically, recombinant kinases were added to the cell-extracted
proteomes to phosphorylate... 
