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  • OER000000222.pdf.jpg
  • Journal article

  • Actin-binding compounds that affect the kinetics of the interaction of cardiac myosin with actin

    • Authors : Roopnarine, Osha; Thomas, David D. (2020)

  • the effects of ten actin binding compounds on the interaction of cardiac myosin subfragment 1 (S1) with pyrene labeled F actin (PFA). These compounds, previously identified from a small-molecule high-throughput screen (HTS), perturb the microsecond structural dynamics of actin and the steady-state activity of actin-activated myosin ATPase. In the present study, we have further characterized their mechanisms of action by measuring their effects on PFA fluorescence,...
  • OER000000920.pdf.jpg
  • Journal article

  • Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin

    • Authors : Oda, Toshiyuki; Yanagisawa, Haruaki; Wakabayashi, Takeyuki (2020)

  • Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct&#...
  • OER000002526.pdf.jpg
  • Journal article

  • Increased actin binding is a shared molecular consequence of numerous spinocerebellar ataxia mutations in β-III-spectrin

    • Authors : Atang, Alexandra E.  (2023)

  • Spinocerebellar ataxia type 5 (SCA5) is a neurodegenerative disease caused by mutations in the SPTBN2 gene encoding the cytoskeletal protein β-III-spectrin. Previously, we demonstrated that a L253P missense mutation, localizing to the β-III-spectrin actin-binding domain (ABD), causes increased actin-binding affinity. Here we investigate the molecular consequences of nine additional ABD-localized, SCA5 missense mutations: V58M, K61E, T62I, K65E, F160C, D255G, T271I, Y272H, and H2...