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  • OER000000813.pdf.jpg
  • Journal article

  • Fast and highly efficient affinity enrichment of Azide-A-DSBSO cross-linked peptides

    • Authors : Matzinger, Manuel; Kandioller, Wolfgang; Doppler, Philipp (2020)

  • Cross-linking mass spectrometry is an increasingly used, powerful technique to study protein-protein interactions or to provide structural information. Due to sub-stochiometric reaction efficiencies, cross-linked peptides are usually low abundant. This results in challenging data evaluation and the need for an effective enrichment. Here we describe an improved, easy to implement, one-step method to enrich azide-tagged, acid-cleavable disuccinimidyl bis-sulfoxide (DSBSO) cross...
  • OER000000773.pdf.jpg
  • Keynote & speech

  • Zirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics

    • Authors : Diez, Ignacio Arribas; Govender, Ireshyn; Naicker, Previn (2020)

  • Phosphopeptide enrichment is an essential step in large-scale, quantitative phosphoproteomics studies by mass spectrometry. Several phosphopeptide affinity enrichment techniques exist, such as Immobilized Metal ion Affinity Chromatography (IMAC) and Metal Oxide Affinity Chromatography (MOAC). We compared Zirconium (IV) IMAC (Zr-IMAC) magnetic microparticles to more commonly used Titanium (IV) IMAC (Ti-IMAC) and TiO2 magnetic microparticles for phosphopeptide enrichment from simple and com...