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  • OER000000863.pdf.jpg
  • Journal article

  • Assessment of mitochondrial physiology of murine white adipose tissue by mechanical permeabilization and lipid depletion

    • Authors : Romeiro, Natália C; Ferreira, Caroline M; Oliveira, Marcus F (2020)

  • White adipose tissue (WAT) is classically associated with energy storage in the form of triacylglycerol and is directly associated with metabolic disorders, including obesity. Mitochondria regulates cellular expenditure and are active in WAT. Although isolated mitochondria have been classically used to assess their functions, several artifacts can be introduced by this approach. Although methods to assess mitochondrial physiology in permeabilized WAT were proposed, importan...
  • OER000000808.pdf.jpg
  • Journal article

  • Functional Changes Induced by Short Term Caloric Restriction in Cardiac and Skeletal Muscle Mitochondria

    • Authors : Serna, Julian David C; Silva, Camille C. Caldeira da; Kowaltowski, Alicia J (2020)

  • Caloric restriction (CR) is widely known to increase life span and resistance against different types of injuries in several organisms. We have previously shown that mitochondria from livers or brains of CR animals exhibit higher calcium uptake rates and lower sensitivity to calcium-induced mitochondrial permeability transition (mPT), an event related to the resilient phenotype exhibited by these organs. Given the importance of calcium in metabolic control an...