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  • OER000002549.pdf.jpg
  • Industry article

  • Anti-CRISPR protein mediated degradation of Cas9 in human cells

    • Authors : Tacca, Luisa Arake de (2023)

  • Bacteriophages encode anti-CRISPR (Acr) proteins that inactivate CRISPR-Cas bacterial8 immune systems, allowing successful invasion, replication, and prophage integration. Acr9 proteins inhibit CRISPR-Cas systems using a wide variety of mechanisms. AcrIIA1 is encoded by10 numerous phages and plasmids, binds specifically to the Cas9 HNH domain, and was the first11 Acr discovered to inhibit SpyCas9. Here we report the observation of AcrIIA1-induced degradation12 ...
  • OER000002533.pdf.jpg
  • Journal article

  • Cooperativity between Cas9 and hyperactive AID establishes broad and diversifying mutational footprints in base editors

    • Authors : Berríos, Kiara N.  (2023)

  • The partnership of DNA deaminase enzymes with CRISPR-Cas nucleases is now a well-established method to enable targeted genomic base editing. However, an understanding of how Cas9 and DNA deaminases collaborate to shape base editor (BE) outcomes has been lacking. Here, we support a novel mechanistic model of base editing by deriving a range of hyperactive activation-induced deaminase (AID) base editors (hBEs) and exploiting their characteristic&#...
  • OER000000866.pdf.jpg
  • Journal article

  • PAM recognition by miniature CRISPR nucleases triggers programmable double-stranded DNA target cleavage

    • Authors : Karvelis, Tautvydas; Bigelyte, Greta; Young, Joshua K (2020)

  • In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer unprecedented flexibility, however, more compact versions would simplify delivery and extend application. Here, we present a collection of 10 exceptionally compact (422-603 amino acids) CRISPR-Cas nucleases that recognize&#x...