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Journal article

Fast and highly efficient affinity enrichment of Azide-A-DSBSO cross-linked peptides

Authors : Matzinger, Manuel; Kandioller, Wolfgang; Doppler, Philipp (2020)

Cross-linking mass spectrometry is an increasingly used, powerful technique to study protein-protein interactions or to provide structural information. Due to sub-stochiometric reaction efficiencies, cross-linked peptides are usually low abundant. This results in challenging data evaluation and the need for an effective enrichment. Here we describe an improved, easy to implement, one-step method to enrich azide-tagged, acid-cleavable disuccinimidyl bis-sulfoxide (DSBSO) cross...

Journal article

Synthesis and Characterisation of Aqueous Haemoglobin-based Microcapsules Coated by Genipin-Cross-Linked Albumin

Authors : Schakowski, Kai Melvin; Linders, Jürgen; Ferenz, Katja Bettina; Kirsch, Michael (2019)

Bovine serum albumin (BSA)-coated haemoglobin (Hb)-microcapsules prepared by co-precipitation of Hb and MnCO3 may present an alternative type of artificial blood substitute. Prepared microcapsules were analysed by Scanning electron microscopy (SEM) and Respirometry, cytotoxicity was evaluated by addition of microcapsules to murine fibroblast-derived cell line L929 (American Type Culture Collection, NCTC clone 929 of strain L). The capsules come along with a mean diameter&#x...