Browse

Author Profile

Browsing by Subject GPCR

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:  
Showing results 1 to 2 of 2
  • OER000000703.pdf.jpg
  • Journal article

  • Molecular Basis for the Evolved Instability of a Human G-Protein Coupled Receptor

    • Authors : Chamness, Laura M; Zelt, Nathan B; Kuntz, Charles P (2020)

  • Membrane proteins are prone to misfolding and degradation. This is particularly true for mammalian forms of the gonadotropin-releasing hormone receptor (GnRHR). Though they function at the plasma membrane, mammalian GnRHRs tend to accumulate within the secretory pathway. Their apparent instability is believed to have evolved in response to selection for attenuated GnRHR activity. Nevertheless, the structural basis of this adaptation remains unclear. We find that t...
  • OER000002393.pdf.jpg
  • Journal Article

  • ThermoBRET: a ligand-engagement nanoscale thermostability assay applied to GPCRs

    • Authors : Hoare, Bradley L. (2023)

  • Measurements of membrane protein thermostability allows indirect detection of ligand 3 binding. Current thermostability assays require protein purification or rely on pre4 existing radiolabelled or fluorescent ligands, limiting their application to established 5 target proteins. Alternative methods detect protein aggregation which requires 6 sufficiently high level of protein expression. 7 Here, we present a ThermoBRET method to quantify the relative thermost...