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Browsing by Subject Glycogen

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Periodicals (Báo – Tạp chí)

A temperature-sensitive Mycobacterium smegmatis glgE mutation leads to a loss of GlgE enzyme activity and thermostability and the accumulation of -maltose-1-phosphate

Authors : Syson, Karl (2020)

To reconstruct and examine the temperature-sensitive mutant and characterise the mutated GlgE enzyme. Results- The mutant strain accumulated the substrate for GlgE, -maltose-1-phosphate, at the non-permissive temperature. The glycogen assay used in the original study was shown to give a false positive result with -maltose-1-phosphate. The accumulation of -maltose-1-phosphate was due to the lowering of the kcat of GlgE as well as a loss of stability 42 ...

Journal article

Lack of astrocytic glycogen alters synaptic plasticity but not seizure susceptibility

Authors : Duran, Jordi; Brewer, M. Kathryn (2020)

Brain glycogen is mainly stored in astrocytes. However, recent studies both in vitro and in vivo indicate that glycogen also plays important roles in neurons. By conditional deletion of glycogen synthase (GYS1), we previously developed a mouse model entirely devoid of glycogen in the central nervous system (GYS1Nestin-KO). These mice displayed altered electrophysiological properties in the hippocampus and increased susceptibility to kainate-induced seizures. To underst...

Journal article

Temperature, but not excess of glycogen, regulate post-mortem AMPK activity in muscle of steer carcasses.

Authors : Strobe, P; Galaz, A; Espíndola, F Villaroel (2020)

That post-mortem AMPK activity level is highly sensitive to temperature and not at in vitro changes in glycogen concentration. Their results suggest that that normal levels of pre-mortem muscle glycogen and an adequate cooling managing of carcasses are relevant to let an efficient glycogenolytic/glycolytic flow required for lactate accumulation and pH decline, trough of post-mortem AMPK signalling pathway.