Browse

Author Profile

Browsing by Subject Lập hồ sơ

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:  
Showing results 1 to 3 of 3
  • OER000002343.pdf.jpg
  • Journal Article

  • Activity-Guided Proteomic Profiling of Proteasomes Uncovers a Variety of Active (And Inactive) Proteasome Species

    • Authors : Sahoo, Manisha Priyadarsini (2023)

  • Proteasomes are multi-subunit, multi-catalytic protein complexes present in eukaryotic cells that degrade misfolded, damaged, or unstructured proteins. In this study, we used an activity-guided proteomic methodology based on a fluorogenic peptide substrate to characterize the composition of proteasome complexes in WT yeast, and the changes these complexes undergo upon the deletion of Pre9 (Δα3) or of Sem1 (ΔSem1). A comparison of whole-cell proteomi...
  • OER000002344.pdf.jpg
  • Journal Article

  • nfluence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles

    • Authors : Gookin, Timothy E. (2023)

  • Proteasomes are multi-subunit, multi-catalytic protein complexes present in eukaryotic cells that degrade misfolded, damaged, or unstructured proteins. In this study, we used an activity-guided proteomic methodology based on a fluorogenic peptide substrate to characterize the composition of proteasome complexes in WT yeast, and the changes these complexes undergo upon the deletion of Pre9 (Δα3) or of Sem1 (ΔSem1). A comparison of whole-cell proteomi...
  • OER000002673.pdf.jpg
  • Journal article

  • System-wide profiling by proteome integral solubility alteration assay of drug residence times for target characterization

    • Authors : Sabatier, Pierre (2023)

  • Most drugs used in the clinic and drug candidates target multiple proteins, and thus detailed characterization of their efficacy targets is required. While current methods rely on quantitative measurements at thermodynamic equilibrium, kinetic parameters such as the residence time of a drug on its target provide a better proxy for efficacy in vivo. Here, we present Residence Time Proteome Integral Solubility Alteration (ResT-PISA) assay which provides monitor...