Browse

Author Profile

Browsing by Subject Lipidomics

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:  
Showing results 1 to 5 of 5
  • OER000000250.pdf.jpg
  • Journal article

  • Aging selectively dampens oscillation of lipid abundance in white and brown adipose tissue

    • Authors : Held, Ntsiki M.; Buijink, M. Renate; Elfrink, Hyung L. (2020)

  • To investigate whether murine white (WAT) and brown (BAT) adipose tissue lipids exhibit rhythmicity and if this is affected by aging. To this end, we have measured the 24 hour lipid profiles of WAT and BAT using a global lipidomics analysis of >1100 lipids. We observed rhythmicity in nearly all lipid classes including glycerolipids, glycerophospholipids, sterol lipids and sphingolipids. Overall, ~22% of the analyzed lipids were considered rhythmic...
  • OER000000372.pdf.jpg
  • Periodicals (Báo – Tạp chí)

  • Lipid extraction from dried blood spots and dried milk spots for high throughput lipidomics

    • Authors : Garmendia, Andres Carrillo; Ortiz, Cecilia Martinez; Garfias, Jairo Getzemani Martinez (2020)

  • Dried blood spots (DBS) and dried milk spots (DMS) represent convenient matrices for collecting and storing human samples. However, the use of these sample types for researching lipid metabolism remains relatively poorly explored, and especially the efficiency of lipid extraction is unclear. A visual inspection of punched DBSs after standard extraction suggests that the samples remain largely intact. DMSs comprise a dense aggregate of milk fat globules o...
  • OER000000764.pdf.jpg
  • Journal article

  • Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteins

    • Authors : Klooster, Joury S van ‘t; Cheng, Tan-Yun; Sikkemaa, Hendrik R (2020)

  • Yeast tolerates a low pH and high solvent concentrations. The permeability of the plasma membrane (PM) for small molecules is low and lateral diffusion of proteins is slow. These findings suggest a high degree of lipid order, which raises the question of how membrane proteins function in such an environment. The yeast PM is segregated into the Micro-Compartment-of-Can1 (MCC) and Pma1 (MCP), which have different lipid compositions. We extracted ...
  • OER000000947.pdf.jpg
  • Journal article

  • Periprotein membrane lipidomics and the role of lipids in transporter function in yeast

    • Authors : Klooster, Joury S van ‘t; Cheng, Tan-Yun; Sikkema, Hendrik R (2020)

  • The yeast plasma membrane is segregated into domains: the Micro-Compartment-of-Can1 (MCC) and Pma1 (MCP) have a different protein composition, but their lipid composition is largely unknown. We extracted proteins residing in these microdomains via stoichiometric capture of lipids and proteins in styrene-maleic-acid-lipid-particles (SMALPs). We purified SMALPs by affinity chromatography and quantitatively analyzed the lipids by mass spectrometry and their role in transporter func...
  • OER000000176.pdf.jpg
  • Periodicals (Báo – Tạp chí)

  • Sphingolipid changes in Parkinson L444P GBA mutation fibroblasts promote α-synuclein aggregation

    • Authors : Galvagnion, Céline (2020)

  • Intraneuronal accumulation of aggregated α-synuclein is a pathological hallmark of Parkinson’s disease. Therefore, mechanisms capable of promoting α-synuclein deposition bear important pathogenetic implications. Mutations of the glucocerebrosidase 1 (GBA) gene represent a prevalent Parkinson’s disease risk factor. They are associated with loss of activity of a key enzyme involved in lipid metabolism, glucocerebrosidase, supporting a mechanistic relationship between abnormal α-synuclein-l...