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Tìm kiếm theo: Chủ đề Mycobacteria

Duyệt theo: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
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Journal article

Fortuitous in vitro compound degradation produces a tractable hit against Mycobacterium tuberculosis dethiobiotin synthetase: a cautionary tale of what goes in, does not always come out

Tác giả : Salaemae, Wanisa (2023)

A molecule flagged in an in silico docking screen against MtDTBS, was inadvertently hydrolysed in the crystal conditions used for hit validation. The resulting fragment-sized molecule bound to the DAPA substrate binding pocket of the target enzyme (MtDTBS) with millimolar affinity, as measured by surface plasmon resonance, but was later modified to a highly potent (nanomolar) ligand and promising lead for the development of novel tuberculos...

Journal article

High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for Mycobacterium tuberculosis RNA polymerase

Tác giả : Jensen, Drake (2023)

The first step in gene expression is the transcription of DNA sequences into RNA. Regulation at the level of transcription leads to changes in steady-state concentrations of RNA transcripts, affecting the flux of downstream functions and ultimately cellular phenotypes. Changes in transcript levels are routinely followed in cellular contexts via genome-wide sequencing techniques. However, in vitro mechanistic studies of transcription have lagged with ...

Journal article

PE5-PPE4-EspG3 trimer structure from mycobacterial ESX-3 secretion system gives insight into cognate substrate recognition by ESX systems

Tác giả : Williamson, Zachary A; Chaton, Catherine T; Ciocca, William A (2020)

Mycobacterium tuberculosis (Mtb) has evolved numerous type VII secretion (ESX) systems to secrete multiple factors important for both growth and virulence across their cell envelope. Three such systems; ESX-1, ESX-3, and ESX-5; have been shown to each secrete a unique set of substrates. A large class of these substrates secreted by these three systems are the PE and PPE families of proteins. Proper secretion of the PE-PPE proteins requires the&...

Journal article

Structural investigation of Mycobacterial protein complexes involved in the stationary phase stress response

Tác giả : Kirykowicz, Angela M; Woodward, Jeremy D (2020)

Large protein complexes play key roles in mediating biological processes in the cell. Little structural information is known on the protein complex mediators governing persistence in the host for Mycobacterium tuberculosis (Mtb). We applied the ‘shotgun EM’ method for the structural characterisation of protein complexes produced after exposure to stationary phase stress for the model Mycobacterium, M smegmatis (Msm). We identified glutamine synthetase I, essential ...