Duyệt theo

Hồ sơ tác giả

Tìm kiếm theo: Chủ đề Proteomics

Duyệt theo: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
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Hiển thị kết quả tìm kiếm từ 1 đến 9 trong 9 kết quả phù hợp
  • OER000000902.pdf.jpg
  • Journal article

  • A mouse SWATH-MS reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts

    • Tác giả : Krasny, Lukas; Bland, Philip; Burns, Jessica (2020)

  • SWATH-mass spectrometry (MS) enables accurate and reproducible proteomic profiling in multiple model organisms including the mouse. Here we present a comprehensive mouse reference spectral library (MouseRefSWATH) that permits quantification of up to 10,597 proteins (62.2% of the mouse proteome) by SWATH-MS. We exploit MouseRefSWATH to develop an analytical pipeline for species-specific deconvolution of proteomic alterations in human tumour xenografts (XenoSWATH). This method ove...
  • OER000002949.pdf.jpg
  • Journal article

  • An experimentally-derived measure of inter-replicate variation in reference samples: the same-same permutation methodology

    • Tác giả : Handler, David C. (2021)

  • The multiple testing problem is a well-known statistical stumbling block in high-25 throughput data analysis, where large scale repetition of statistical methods introduces 26 unwanted noise into the results. While approaches exist to overcome the multiple testing 27 problem, these methods focus on theoretical statistical clarification rather than incorporating 28 experimentally-derived measures to ensure appropriately tailored analysis parameters. Here, 29 we introduce a m...
  • OER000002663.pdf.jpg
  • Journal article

  • Chemical Proteomics Reveals Protein Tyrosination Extends Beyond the Alpha-Tubulins in Human Cells

    • Tác giả : Makarov, Dmytro  (2023)

  • Tubulin detyrosination-tyrosination cycle regulates the stability of microtubules. Thus far described on α-tubulins, the tyrosination level is maintained by a single tubulin-tyrosine ligase (TTL). However, the precise dynamics and tubulin isoforms which undergo (de)tyrosination in neurons are unknown. Here, we exploit the substrate promiscuity of the TTL to introduce an O-propargyl-L-tyrosine in neuroblastoma cells and neurons. Mass spectrometry-based chemical proteomics in neuroblas...
  • OER000000689.pdf.jpg
  • Journal article

  • Erosion of human X chromosome inactivation causes major remodelling of the iPSC proteome

    • Tác giả : Brenes, Alejandro J; Yoshikawa, Harunori; Bensaddek, Dalila (2020)

  • X chromosome inactivation (XCI) is a dosage compensation mechanism in female mammals whereby genes from one X chromosome are repressed. Analysis of human induced pluripotent stem cell (iPSC) lines using proteomics, RNAseq and polysome profiling showed a major change in the proteome upon XCI erosion. This resulted in amplified RNA and protein expression from X-linked genes. However, increased protein expression was also detected from autosomal genes without&#x...
  • OER000000741.pdf.jpg
  • Journal article

  • In silico approach toward the identification of unique peptides from viral protein infection: Application to COVID-19

    • Tác giả : Orsburn, Benjamin C; Jenkins, Conor; Miller, Sierra M (2020)

  • We describe a method for rapid in silico selection of diagnostic peptides from newly described viral pathogens and applied this approach to SARS-CoV-2/COVID-19. This approach is multi-tiered, beginning with compiling the theoretical protein sequences from genomic derived data. In the case of SARS-CoV-2 we begin with 496 peptides that would be produced by proteolytic digestion of the viral proteins. To eliminate peptides that would cause cross-reactivity ...
  • OER000000792.pdf.jpg
  • Journal article

  • Integrated metabolomics and proteomics of symptomatic and early pre-symptomatic states of colitis

    • Tác giả : Shimshoni, Elee; Ghini, Veronica; Solomonov, Inna (2020)

  • Two murine models for colitis were used to study multi-level changes and derive molecular signatures of colitis onset and development. By combining metabolomics data on tissues and fecal extracts with proteomics data on tissues, we provide a comprehensive picture of the metabolic profile of acute and chronic states of the disease, and most importantly, of two early pre-symptomatic states. We show that, increased anaerobic glycolysis, accompanied by alter...
  • OER000002667.pdf.jpg
  • Journal article

  • Proteomics and metabolomics reveal an abundant α-glucosidase drives sorghum fermentability for beer brewing

    • Tác giả : Kerr, E. D. (2023)

  • Sorghum (Sorghum bicolor), a grass native to Africa, is a popular alternative to barley for brewing beer. The importance of sorghum to beer brewing is increasing because it is a naturally gluten-free cereal and because climate change is expected to cause a reduction in the production of barley over the coming decades. However, there are challenges associated with the use of sorghum instead of barley in beer brewing. Here,...
  • OER000000940.pdf.jpg
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  • Solid-Phase Peptide Capture and Release for Bulk and Single-Molecule Proteomics

    • Tác giả : Howard, Cecil J; Floyd, Brendan M; Bardo, Angela M (2020)

  • The field of proteomics has expanded recently with more sensitive techniques for the bulk measurement of peptides as well as single-molecule techniques. One limiting factor for some of these methods is the need for multiple chemical derivatizations and highly pure proteins free of contaminants. We demonstrate a solid-phase capture strategy suitable for the proteolysis, purification, and subsequent chemical modification of peptides. We use this resin on an...