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Browsing by Subject RNA modifications

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  • OER000000911.pdf.jpg
  • Journal article

  • Deoxyribozyme-based Method for Site-specific Absolute Quantification of N6-methyladenosine Modification Fraction

    • Authors : Bujnowska, Magda; Zhang, Jiacheng; Dai, Qing (2020)

  • N6-methyladenosine (m6A) is the most prevalent modified base in eukaryotic messenger RNA (mRNA) and long noncoding RNA (lncRNA). Although candidate sites for m6A modification are identified at the transcriptomic level, site-specific quantification methods for m6A modifications are still limited. Herein, we present a facile method implementing deoxyribozyme that preferentially cleaves the unmodified RNA. We leverage reverse transcription and real-time quantitative PCR along with&...
  • OER000000924.pdf.jpg
  • Journal article

  • Production and Purification of Endogenously Modified tRNA-Derived Small RNAs

    • Authors : Drino, Aleksej; Oberbauer, Vera; Troger, Conor (2020)

  • During particular stress conditions, transfer RNAs (tRNAs) become substrates of stress-induced endonucleases, resulting in the production of distinct tRNA-derived small RNAs (tsRNAs). These small RNAs have been implicated in a wide range of biological processes, but how isoacceptor and even isodecoder-specific tsRNAs act at the molecular level is still poorly understood. Importantly, stress-induced tRNA cleavage affects only a few tRNAs of a given isoacceptor or i...