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Browsing by Subject RNA polymerase

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  • OER000000917.pdf.jpg
  • Journal article

  • Chemical transcription roadblocking for nascent RNA display

    • Authors : Strobel, Eric J; Lis, John T; Lucks, Julius B (2020)

  • Site-specific arrest of RNA polymerase is fundamental to several technologies that measure RNA structure and function. Current in vitro transcription ‘roadblocking’ approaches inhibit transcription elongation using a protein blockade bound to the DNA template. One limitation of protein-mediated transcription roadblocking is that it requires the inclusion of a protein factor that is extrinsic to the minimal in vitro transcription reaction. In this work, we show tha...
  • OER000002468.pdf.jpg
  • Journal Article

  • Pathogenic bacteria experience pervasive RNA polymerase backtracking during infection

    • Authors : Browning, Kaitlyn R. (2023)

  • Pathogenic bacteria and their eukaryotic hosts are in a constant arms race. Hosts have numerous defense mechanisms at their disposal that not only challenge the bacterial invaders, but have the potential to disrupt molecular transactions along the bacterial chromosome. However, it is unclear how the host impacts association of proteins with the bacterial chromosome at the molecular level during infection. This is partially due to the lack of a ...
  • OER000002741.pdf.jpg
  • Journal article

  • Three-dimensional envelope and subunit interactions of the plastid-encoded RNA polymerase from Sinapis alba

    • Authors : Ruedas, Rémi (2023)

  • RNA polymerases (RNAPs) involved in gene transcription are found in all living organisms with degrees of complexity ranging from single polypeptide chains to multimeric enzymes. In the chloroplasts, the nuclear-encoded RNA polymerase and the plastid-encoded RNA polymerase (PEP) are both involved in the selective transcription of the plastid genome. The PEP is a prokaryotic-type multimeric RNAP found in different states depending on light stimuli ...