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Journal article

Adaptation of the binding domain of Lactobacillus acidophilus S-layer protein as a molecular tag for affinity chromatography development

Authors : Muruaga, Emanuel J. (2023)

The SLAP TAG is a novel molecular TAG derived from a protein domain present in the sequence of22 Lactobacillus acidophilus SlpA (SlpA284-444 ). Proteins from different biological sources, with different23 molecular weights or biochemical functions, can be fused in frame to the SLAP TAG and efficiently purified24 by the specific binding to a bacterial-derived chromatographic matrix named here Bio-Matrix (BM).25 Different binding and elution ...

Journal article

Adaptation of the binding domain of Lactobacillus acidophilus S-layer protein as a molecular tag for affinity chromatography development

Authors : Muruaga, Emanuel J. (2023)

The SLAPTAG is a novel molecular TAG derived from a protein domain present in the sequence of 22 Lactobacillus acidophilus SlpA (SlpA284-444). Proteins from different biological sources, with different 23 molecular weights or biochemical functions, can be fused in frame to the SLAPTAG and efficiently purified 24 by the specific binding to a bacterial-derived chromatographic matrix named here Bio-Matrix (BM). 25 Different binding and elution conditions were&#x...