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  • OER000000170.pdf.jpg
  • Periodicals (Báo – Tạp chí)

  • Amphipathic Fluorescent Dyes for Sensitive and Long-Term Monitoring of Plasma Membranes

    • Authors : Daly, Leonard (2020)

  • Adaption of cells to low oxygen environments is an essential process mediated in part by the Hypoxia Inducible Factors (HIFs). Like other transcription factors, the stability and transcriptional activity of HIFs, and consequently he hypoxic response, are regulated by post-translational modification (PTM) and changes in biomolecular interactions. However, our current understanding of PTM-mediated regulation of HIFs is primarily based on in vitro protein fragment-based s...
  • OER000000917.pdf.jpg
  • Journal article

  • Chemical transcription roadblocking for nascent RNA display

    • Authors : Strobel, Eric J; Lis, John T; Lucks, Julius B (2020)

  • Site-specific arrest of RNA polymerase is fundamental to several technologies that measure RNA structure and function. Current in vitro transcription ‘roadblocking’ approaches inhibit transcription elongation using a protein blockade bound to the DNA template. One limitation of protein-mediated transcription roadblocking is that it requires the inclusion of a protein factor that is extrinsic to the minimal in vitro transcription reaction. In this work, we show tha...
  • OER000000685.pdf.jpg
  • Journal article

  • Detect Genomic G-Quadruplexes in Living Animal Cells with a Tiny Artificial Protein Probe

    • Authors : Zheng, Ke-wei; Zhang, Jia-yu; He, Yi-de (2020)

  • G-quadruplex (G4) structures formed by guanine-rich nucleic acids are implicated in essential physiological processes and serve as important drug targets. The genome-wide detection of G4s in living cells is important for exploring the biological role of G4s but has not yet been achieved due to the lack of a suitable G4 probe. We engineered a 6.7 kDa G4 probe (G4P) protein that binds G4s with high affinity and specificity. We used it&#...