Journal articleAuthors : Nierves, Lorenz (2021)
The high affinity of biotin to streptavidin has made it one of the most widely used
affinity tags in proteomics. Early methods used biotin for enrichment alone and mostly
ignored the biotin labeled peptide. Recent advances in labeling led to an increase in
biotinylation efficiency and shifted the interest to detection of the site of biotinylation.
This increased confidence in identification and provides additional structural information...