Browse

Author Profile

Browsing by Subject cơ chất

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:  
Showing results 1 to 11 of 11
  • OER000002335.pdf.jpg
  • Journal Article

  • Algal kainoid synthases exhibit substrate-dependent hydroxylation and cyclization activities

    • Authors : Hopiavuori, Austin R. (2023)

  • FeII/a-ketoglutarate-dependent dioxygenases (Fe/aKG) are a large enzyme family that functionalize C-H bonds on diverse organic substrates. Although Fe/aKG homologs catalyze an array of chemically useful reactions, hydroxylation typically predominates. Microalgal DabC uniquely forms a novel C-C bond to construct the bioactive pyrrolidine ring in domoic acid biosynthesis. However, this kainoid synthase exclusively performs a stereospecific hydroxylation reaction on it...
  • OER000002573.pdf.jpg
  • Journal article

  • Enzymatic assay for UDP-GlcNAc and its application in the parallel assessment of substrate availability and protein O-GlcNAcylation

    • Authors : Sunden, Marc (2023)

  • O-linked N-acetylglucosaminylation (O-GlcNAcylation) is a ubiquitous and dynamic yet still relatively poorly understood non-canonical glycosylation of intracellular proteins. Several vital branches of metabolism converge at the hexosamine biosynthetic pathway (HBP) to produce the substrate for protein O-GlcNAcylation the uridine diphosphate N-acetylglucosamine (UDP-GlcNAc). Availability of this metabolite is considered a key regulator of O-GlcNAcylation. Yet UDP-GlcNAc concentrations ...
  • OER000002503.pdf.jpg
  • Journal article

  • Identification of a new Corin atrial natriuretic peptide-converting enzyme substrate: Agouti-signaling protein (ASIP)

    • Authors : MacDonald, Bryan T.  (2023)

  • Corin is a transmembrane tethered enzyme best known for processing the hormone atrial natriuretic peptide (ANP) in cardiomyocytes to control electrolyte balance and blood pressure. Loss of function mutations in Corin prevent ANP processing and lead to hypertension. Curiously, Corin loss of function variants also result in lighter coat color pigmentation in multiple species. Corin pigmentation effects are dependent on a functional Agouti locus encoding the ago...
  • OER000002672.pdf.jpg
  • Journal article

  • Low normal factor V enhances thrombin generation in hemophilia A by a substrate competition mechanism with factor Xa

    • Authors : Monroe, Dougald M. (2023)

  • Bleeding patterns in people with hemophilia A cannot be predicted solely by factor VIII (FVIII) levels. Some of the variance in bleeding may be attributed to differences in plasma protein composition, and specifically other coagulation factors where the normal ranges span 50-150% of the population mean. We recently used a mathematical model of thrombus formation that identified factor V (FV) levels as a strong modifier of thrombin ...
  • OER000003047.pdf.jpg
  • Journal article

  • Reconciling Conformational Heterogeneity and Substrate Recognition in Cytochrome P450

    • Authors : Dandekar, B. (2021)

  • Cytochrome P450, the ubiquitous metalloenzyme involved in detoxification of foreign components, has remained one of the most popular systems for substrate-recognition process. However, despite being known for its high substrate specificity, the mechanistic basis of substrate-binding by archetypal system cytochrome P450cam has remained at odds with the contrasting reports of multiple diverse crystallographic structures of its substrate-free form. Here we address this...
  • OER000002324.pdf.jpg
  • Journal Article

  • tRNA m1G9 modification depends on substrate-specific RNA conformational changes induced by the methyltransferase Trm10

    • Authors : E strassler, sarah (2023)

  • The methyltransferase Trm10 modifies a subset of tRNAs on the base N1 position of the 9th nucleotide in the tRNA core. Trm10 is conserved throughout Eukarya and Archaea, and mutations in the human gene (TRMT10A) have been linked to neurological disorders such as microcephaly and intellectual disability, as well as defects in glucose metabolism. Of the 26 tRNAs in yeast with guanosine at position 9, only 14 are substrates ...
  • OER000002648.pdf.jpg
  • Journal article

  • Universal Design Principle to Enhance Enzymatic Activity using the Substrate Affinity

    • Authors : Ooka, Hideshi (2023)

  • Design principles to improve enzymatic activity are essential to promote energy-material conversion using biological systems. For more than a century, the Michaelis-Menten equation has provided a fundamental framework of enzymatic activity. However, there is still no concrete guideline on how the parameters should be optimized to enhance enzymatic activity. Here, we demonstrate that tuning the Michaelis-Menten constant (𝐾𝑚) to the substrate concentration (𝑆) maximizes&#x...
  • OER000002359.pdf.jpg
  • Journal Article

  • Validity of the cell-extracted proteome as a substrate pool for exploring phosphorylation motifs of kinases

    • Authors : Ishihama, Yasushi (2023)

  • Three representative protein kinases with different substrate preferences, ERK1 (Prodirected), CK2 (acidophilic), and PKA (basophilic), were used to investigate phosphorylation sequence motifs in substrate pools consisting of the proteomes from three different cell lines, MCF7 (human mammary carcinoma), HeLa (human cervical carcinoma), and Jurkat (human acute T-cell leukemia). Specifically, recombinant kinases were added to the cell-extracted proteomes to phosphorylate...