Duyệt theo

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Duyệt theo: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
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  • Journal article

  • Antisense pairing and SNORD13 structure guide RNA cytidine acetylation

    • Tác giả : Gamage, Supuni Thalalla (2023)

  • N4-acetylcytidine (ac4C) is an RNA nucleobase found in all domains of life. Establishment of ac4C in helix 45 (h45) of human 18S ribosomal RNA (rRNA) requires the combined activity of the acetyltransferase NAT10 and the box C/D snoRNA SNORD13. However, the molecular mechanisms governing RNA-guided nucleobase acetylation in humans remain unexplored. Here we report two assays that enable the study of SNORD13-dependent RNA acetylation in human cells. F...
  • OER000002299.pdf.jpg
  • Journal Article

  • Application of monolayer graphene to cryo-electron microscopy grids for high-resolution structure determination

    • Tác giả : Grassetti, andrew V (2023)

  • The application of support layers, such as graphene, to cryo-electron microscopy grids can increase the density of particles imaged, limit particle interactions with the air-water interface, reduce the extent of beam-induced motion, and, in some instances, improve the distribution of particle orientations. This paper describes a robust protocol for coating cryo-EM grids with a monolayer of graphene for improved cryo-sample preparation. Single particle cryo...
  • OER000002709.pdf.jpg
  • Journal article

  • Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro

    • Tác giả : Yadav, Ruchi (2023)

  • SARS-CoV-2, a human coronavirus, is the causative agent of the COVID-19 34 pandemic. Its ~30 kb RNA genome is translated into two large polyproteins subsequently cleaved 35 by viral papain-like protease and main protease (Mpro/nsp5). Polyprotein processing is essential 36 yet incompletely understood. We studied Mpro-mediated processing of the nsp7-10/11 37 polyprotein, whose mature products are cofactors of the viral replicase, identifying the&#...
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  • Journal Article

  • Cardiomyopathy-Associated Variants Alter the Structure and Function of the α-Actinin-2 Actin-Binding Domain

    • Tác giả : E Atang, Alexandra (2023)

  • Hypertrophic cardiomyopathy (HCM), dilated cardiomyopathy (DCM), and restrictive cardiomyopathy (RCM) are characterized by thickening, thinning, or stiffening, respectively, of the ventricular myocardium, resulting in diastolic or systolic dysfunction that can lead to heart failure and sudden cardiac death. Recently, variants in the ACTN2 gene, encoding the protein α-actinin-2, have been reported in HCM, DCM, and RCM patients. However, functional data supporting the pathogenicit...
  • OER000002661.pdf.jpg
  • Journal article

  • Conserved Structure Modules within the lncRNA SChLAP1 Mediate Protein Recognition Implicated in Aggressive Prostate Cancer

    • Tác giả : McFadden, Emily J. (2023)

  • The lncRNA Second Chromosome Locus Associated with Prostate 1 (SChLAP1) was previously identified as a predictive biomarker and potential driver of aggressive prostate cancer. Recent work suggests that SChLAP1 may bind the SWI/SNF chromatin remodeling complex to promote prostate cancer metastasis, though the exact role of SWI/SNF recognition is debated. To date, there are no detailed biochemical studies of apo SChLAP1 or SChLAP1:protein com...
  • OER000003039.pdf.jpg
  • Journal article

  • Considerations on activity determinants of fungal polyphenol oxidases based on mutational and structural studies

    • Tác giả : Nikolaivits, Efstratios (2021)

  • Polyphenol oxidases (PPOs) are an industrially relevant family of enzymes, being involved in the post-harvest browning of fruits and vegetables, as well as in human melanogenesis. Their involvement lies in their ability to oxidize phenolic or polyphenolic compounds, that subsequently form pigments. PPO family includes tyrosinases and catechol oxidases, which in spite of their high structural similarity, exhibit different catalytic activities. Long-standing research efforts&...
  • OER000002611.pdf.jpg
  • Journal article

  • Lipid nanodiscs as a template for high-resolution cryo-EM structures of peripheral membrane proteins

    • Tác giả : Cannon, Kevin S. (2023)

  • processes such as signal transduction, membrane trafficking, and autophagy. Transient binding to the membrane has a profound impact on protein function, serving to induce conformational changes and alter biochemical and biophysical parameters by increasing the local concentration of factors and restricting diffusion to two dimensions. Despite the centrality of the membrane in serving as a template for cell biology, there are few reported highresolution stru...
  • OER000002959.pdf.jpg
  • Journal article

  • Nanopore dwell time analysis permits sequencing and conformational assignment of pseudouridine in SARS-CoV-2

    • Tác giả : Fleming, Aaron M. (2021)

  • Nanopore devices can directly sequence RNA, and the method has the potential to determine locations of epitranscriptomic modifications that have grown in significance because of their roles in cell regulation and stress response. Pseudouridine (Ψ), the most common modification in RNA, was sequenced with a nanopore system using a protein sensor with a helicase brake in synthetic RNAs with 100% modification at 18 known human pseudouridinylati...
  • OER000002772.pdf.jpg
  • Journal article

  • Sensitive detection and structural characterisation of UV-induced cross-links in protein-RNA complexes using CLIR-MS

    • Tác giả : Sarnowski, Chris P. (2023)

  • Cross-linking coupled with mass spectrometry is an increasingly popular methodology for elucidating structural information from biological complexes. Whilst protein-protein cross-linking workflows are widely used and well characterised, adoption of protein-RNA cross-linking workflows for structural studies is less widespread, and data produced from such experiments remains less well understood. The cross-linking of stable isotope labelled RNA coupled to mass spectrometry (CLIR-MS) workflo...
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  • Journal Article

  • Shared Structural Features of Miro Binding Control Mitochondrial Homeostasis

    • Tác giả : Covill-Cooke, Christian (2023)

  • Miro proteins are universally conserved mitochondrial calcium-binding GTPases that regulate a multitude of mitochondrial processes, including transport, clearance and lipid trafficking. Miro binds a variety of client proteins involved in these functions. How this binding is operated at the molecular level and whether and how it is important for mitochondrial health, however, remains unknown. Here, we show that known Miro clients all use a similar short motif&...
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  • Industry article

  • Structural and mechanistic insights into activation of the human RNA ligase RTCB by Archease

    • Tác giả : gerber, janina Lara (2023)

  • RNA ligases of the RTCB-type play an essential role in tRNA splicing, the unfolded protein response and RNA repair. RTCB is the catalytic subunit of the pentameric human tRNA ligase complex. RNA ligation by the tRNA-ligase complex requires GTP-dependent activation of RTCB. This active site guanylylation reaction relies on the activation factor Archease. The mechanistic interplay between both proteins has remained unknown. Here, we report a&...
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  • Journal article

  • Structural and Preliminary Biochemical Characterization of MppQ, a PLP-Dependent Aminotransferase from Streptomyces hygroscopicus

    • Tác giả : Vuksanovic, Nemanja (2023)

  • MppQ is an enzyme of unknown function from Streptomyces hygroscopicus that is involved in the biosynthesis of the nonproteinogenic amino acid L-enduracididine (L-End). Since L-End is a component of several peptides showing high activity against methicillin-resistant Staphylococcus aureus (MRSA), a complete understanding of its biosynthetic pathway is of utmost importance for developing chemoenzymatic routes for syntheses of novel antibiotics. In this work, we report hi...
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  • Ebooks (Sách điện tử)

  • Structural Characterization of the Full-length Hantaan Virus Polymerase

    • Tác giả : keown, Jeremy R (2023)

  • Hantaviridae are a family of segmented negative-sense RNA viruses that contains important human and animal pathogens including Andes orthohantavirus and Hantaan orthohantavirus. Hantaviridae contain a viral RNA-dependent RNA polymerase that replicates and transcribes the viral genome in the cytoplasm of infected cells. Here we establish the expression and purification of the polymerase from the Old World Hantaan orthohantavirus and characterise the structure&#...
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  • Journal article

  • Structural insights into agonist-binding and activation of the human complement C3a receptor

    • Tác giả : Yadav, Manish K. (2023)

  • The complement cascade is an integral part of innate immunity, and it plays a crucial role in our body's innate immune response including combating microbial infections. Activation of the complement cascade results in the generation of multiple peptide fragments, of which complement C3a and C5a are potent anaphylatoxins. The complement C3a binds and activates a G protein-coupled receptor (GPCR) known as C3aR while C5a activates two distinct receptors...