Ebooks (Sách điện tử)Authors : Hehl, Laura A. (2023)
Ubiquitin chain formation by HECT catalytic domain-containing E3 ligases regulates vast
biology, yet the structural mechanisms remain unknown. We employed chemistry and cryo-
EM to visualize stable mimics of the intermediates along K48-linked ubiquitin chain formation
by the human E3, UBR5. The structural data reveal a ≈620 kDa UBR5 dimer as the
functional unit, comprising a scaffold with flexibly-tethered ubiquitin-binding UBA domains,
and ela... 
