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Journal article

Increasing proteome depth while maintaining quantitative precision in short gradient data-independent acquisition proteomics

Authors : Doellinger, Joerg (2023)

The combination of short liquid chromatography (LC) gradients and data independent acquisition (DIA) by mass spectrometry (MS) has proven its huge potential for high-throughput proteomics. This methodology benefits from the speed of the latest generation of mass spectrometers, which enable short MS cycle times needed to provide sufficient sampling of sharp LC peaks. However, the optimization of isolation window schemes resulting in a certain num...