Journal articleAuthors : Dodge, Greg J. (2023)
Phosphoglycosyl transferases (PGTs) are among the first membrane-bound enzymes involved in the
biosynthesis of bacterial glycoconjugates. Robust expression and purification protocols for an abundant
subfamily of PGTs remains lacking. Recent advancements in detergent-free methods for membrane
protein solubilization open the door for purification of difficult membrane proteins directly from cell
membranes into native-like liponanoparticles. By leveraging autoinduction, in vivo...