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Showing results 1 to 4 of 4
  • OER000003018.pdf.jpg
  • Journal article

  • A novel heteromeric pantothenate kinase complex in apicomplexan parasites

    • Authors : Tjhin, Erick T. (2021)

  • Coenzyme A is synthesised from pantothenate via five enzyme-mediated steps. The first step is catalysed by pantothenate kinase (PanK). All PanKs characterised to date form homodimers. Many organisms express multiple PanKs. In some cases, these PanKs are not functionally redundant, and some appear to be non-functional. Here, we investigate the PanKs in two pathogenic apicomplexan parasites, Plasmodium falciparum and Toxoplasma gondii. Each of these organisms expres...
  • OER000002476.pdf.jpg
  • Journal Article

  • Exposing the molecular heterogeneity of glycosylated biotherapeutics

    • Authors : Schachner, Luis F. (2023)

  • Glycosylated biotherapeutics are an emerging class of drugs with high molecular heterogeneity, which can affect their safety and efficacy. Characterizing this heterogeneity is crucial for drug development and quality assessment, but existing methods are limited in their ability to analyze intact glycoproteins. Here, we present a new approach to glycoform fingerprinting that uses proton-transfer charge-reduction with gas-phase fractionation to analyze intact&#...
  • OER000002590.pdf.jpg
  • Journal article

  • Lipid signatures and inter-cellular heterogeneity of naïve and lipopolysaccharide-stimulated human microglia-like cells

    • Authors : Müller, Max A. (2023)

  • Microglia are non-neuronal cells, which are residing in the central nervous system and are known to play an important role in health and disease. We investigated lipidomic phenotypes of human naïve and stimulated microglia-like cells by atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI). With lateral resolutions between 5 μm and 1.5 μm, we were able to chart lipid compositions of individua...
  • OER000003047.pdf.jpg
  • Journal article

  • Reconciling Conformational Heterogeneity and Substrate Recognition in Cytochrome P450

    • Authors : Dandekar, B. (2021)

  • Cytochrome P450, the ubiquitous metalloenzyme involved in detoxification of foreign components, has remained one of the most popular systems for substrate-recognition process. However, despite being known for its high substrate specificity, the mechanistic basis of substrate-binding by archetypal system cytochrome P450cam has remained at odds with the contrasting reports of multiple diverse crystallographic structures of its substrate-free form. Here we address this...