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Showing results 1 to 9 of 9

Journal article

A targeted isotope dilution mass spectrometry assay for Osteopontin quantification in plasma of metastatic breast cancer patients

Authors : Leslie, Andrew (2023)

Osteopontin (OPN) is a secreted glycophosphoprotein that derives its name from its high abundance in 33 bone and secretion by osteoblasts. It is also secreted by a number of immune cells and, therefore, is 34 present in human plasma at nanogram per millilitre levels where it affects cell adhesion and motility. 35 These properties implicate OPN as a promotor of tumor progression. Plasma OPN is primarily measured 36 by enz...

Journal Article

Cross-linking mass spectrometric analysis of the endogenous TREX complex from S. cerevisiae

Authors : Kern, Carina (2023)

The conserved TREX complex has multiple functions in gene expression such as transcription elongation, 3’ end processing, mRNP assembly and nuclear mRNA export as well as the maintenance of genomic stability. In S. cerevisiae, TREX is composed of the pentameric THO complex, the DEAD-box RNA helicase Sub2, the nuclear mRNA export adaptor Yra1 and the SR-like proteins Gbp2 and Hrb1. Here, we present the structural analysis of the&#x...

Journal article

Cross-species proteomic and microRNA comparison of extracellular vesicles in human milk, cow’s milk, and infant formula products: moving towards next generation infant formula products

Authors : Turner, Natalie P. (2023)

Milk and milk products such as infant formula (IF) play a fundamental role in serving the nutritional 15 needs of the developing infant. Extracellular vesicles (EVs) in human (HM) and cow’s milk (CM) contain 16 molecular cargo such as proteins and micro(mi)RNA that serve as functional messengers between cells 17 and may be of importance to infant health. Here, we have developed a pipeline using advanced 18 proteomics and transcriptomics to...

Journal Article

Label-free quantification LC-mass spectrometry proteomic analysis of blood plasma in healthy dogs

Authors : Doulidis, Pavlos G. (2023)

Bloodwork is a widely used diagnostic tool in veterinary medicine, as diagnosis and therapeutic 15 interventions often rely on blood biomarkers. However, biomarkers available in veterinary medicine 16 often lack sensitivity or specificity. Mass spectrometry (MS)-based proteomics technology has been 17 extensively used in biological fluids and offers excellent potential for a more comprehensive 18 characterization of the plasma proteome in veterinary medicine. In this&#...

Journal article

Mass Spectrometry Reveals that Oxysterols are Secreted from Non-Alcoholic Fatty Liver Disease Induced Organoids

Authors : Kømurcu, Kristina Sæterdal (2023)

Oxysterols are potential biomarkers for liver metabolism that are altered under disease 13 conditions such as non-alcoholic fatty liver disease (NAFLD). We here apply sterolomics to 14 organoids used for disease modeling of NAFLD. Using liquid chromatography-mass spectrometry 15 with on-line sample clean-up and enrichment, we establish that liver organoids produce and 16 secrete oxysterols. We find elevated levels of 26-hydroxycholesterol, an LXR ago...

Journal article

Native size exclusion chromatography-based mass spectrometry (SEC-MS) identifies novel components of the Heat Shock Protein 90-dependent proteome

Authors : Samant, Rahul S. (2023)

works in concert with co-chaperones to stabilize its client proteins, which include multiple drivers of oncogenesis and malignant progression. Pharmacologic inhibitors of HSP90 have been observed to exert a wide range of effects on the proteome, including depletion of client proteins, induction of heat shock proteins, dissociation of co-chaperones from HSP90, disruption of client protein signaling networks, and recruitment of the protein ...

Journal Article

Reverse engineering the anti-MUC1 hybridoma antibody 139H2 by mass spectrometry-based de novo sequencing

Authors : Peng, Weiwei (2023)

Mucin 1 (MUC1) is a transmembrane mucin expressed at the apical surface of epithelial cells at different mucosal surfaces including breast and intestine. In the gastrointestinal tract, MUC1 has a barrier function against bacterial invasion, but can also serve as an entry receptor for pathogenic Salmonella bacteria. Moreover, MUC1 is well known for its aberrant expression and glycosylation in adenocarcinomas The MUC1 extracellular domain contains a variable&#x...

Journal article

Species identification of early colonial bone artefacts excavated from Pyrmont, Australia, by mass spectrometric identification of collagen peptides

Authors : Multari, Dylan H. (2023)

Zooarchaeology by Mass Spectrometry (ZooMS) is a rapidly developing and increasingly 21 utilised peptide mass fingerprinting (PMF) technique that analyses Collagen 1A1 and 1A2 22 marker peptides for the genus- or species-level identification of fragmentary bones in the 23 archaeological record. Traditionally, this analysis is performed using matrix-assisted laser 24 desorption/ionisation time-of-flight mass spectrometry (MALDI-ToF-MS) to identify 25 characteristic m/z values...

Journal article

Structural probing of Hsp26 activation and client binding by quantitative cross-linking mass spectrometry

Authors : Fürsch, Julius (2021)

Small heat-shock proteins (sHSP) are important members of the cellular stress response in all species. Their best described function is the binding of early unfolding states and the resulting prevention of protein aggregation. Most sHSPs exist as oligomers but vary in size and subunit organization. Many sHSPs exist as a polydisperse composition of oligomers which undergoes changes in subunit composition, folding status and rel...