Journal articleAuthors : Roy, Rahul (2023)
Amplification-based qPCR provides accurate and sensitive nucleic acid quantification.
However, the requirement of temperature cycling and real-time monitoring
limits its translation to different settings. Here, we adapted isothermal Recombinase
Polymerase Amplification (RPA) reaction to develop a semi-quantitative method that
relies on final amplicon yield to estimate initial target nucleic acid copy number. To
achieve this, we developed a phenomenological model&#... 
