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Showing results 1 to 6 of 6

Journal article

A systematic structural comparison of all solved small proteins (4-6 kDa) reveals the weight of disulfide bonds in proteins’ foldability

Authors : Moreira, Mariana H. (2021)

Defensins are small proteins, usually ranging from 4 to 6 kDa, amphipathic, disulfide rich, and with a small or even absent hydrophobic core. Since a hydrophobic core is generally found in globular proteins that fold in an aqueous solvent, the peculiar fold of defensins can challenge tertiary protein structure predictors. We performed a PDB-wide survey of small proteins (4-6 kDa) to understand the similarities of defensins ...

Journal article

Adaptation of the binding domain of Lactobacillus acidophilus S-layer protein as a molecular tag for affinity chromatography development

Authors : Muruaga, Emanuel J. (2023)

The SLAP TAG is a novel molecular TAG derived from a protein domain present in the sequence of22 Lactobacillus acidophilus SlpA (SlpA284-444 ). Proteins from different biological sources, with different23 molecular weights or biochemical functions, can be fused in frame to the SLAP TAG and efficiently purified24 by the specific binding to a bacterial-derived chromatographic matrix named here Bio-Matrix (BM).25 Different binding and elution ...

Journal article

G protein βγ subunits bind to and inhibit the function of multiple Qa- and Qb,c-SNARE isoforms

Authors : Zurawski, Zack (2023)

While the ability of G protein βγ subunits (Gβγ) to bind to and functionally inhibit the neuronal SNARE proteins Stx1A, SNAP25, and synaptobrevin in the presence of the calcium sensor synaptotagmin I is well documented, these three SNARE proteins, which form the core SNARE complex for synchronous evoked release in neurons, are but a subset of the larger family of SNARE proteins, which participate in many other exocytic proces...

Ebooks (Sách điện tử)

Structure of puromycin-sensitive aminopeptidase and polyglutamine binding

Authors : Madabushi, Sowmya (2023)

Puromycin-sensitive aminopeptidase (E.C. 3.4.11.14, UniProt P55786), a zinc 6 metallopeptidase belonging to the M1 family, degrades a number of bioactive peptides as 7 well as peptides released from the proteasome, including polyglutamine. We report the 8 crystal structure of PSA at 2.3 Ǻ. Overall, the enzyme adopts a V-shaped architecture 9 with four domains characteristic of the M1 family aminopeptidases, but it is in a less 10...

Journal article

Structure of the human Bre1 complex bound to the nucleosome

Authors : Onishi, Shuhei (2023)

Histone H2B monoubiquitination (at Lys120 in humans) regulates transcription elongation and DNA repair. In humans, H2B monoubiquitination is catalyzed by the heterodimeric Bre1 complex composed of Bre1A/RNF20 and Bre1B/RNF40. The Bre1 proteins generally function as tumor suppressors, while in certain cancers, they facilitate cancer cell proliferation. To reveal the structural basis of H2BK120 ubiquitination and its regulation, we determined the cryo-EM structure of...

Journal article

Transcription initiation at a consensus bacterial promoter proceeds via a “bind-unwind-load-and-lock” mechanism

Authors : Mazumder, Abhishek (2021)

Transcription initiation starts with unwinding of promoter DNA by RNA polymerase (RNAP) to form a catalytically competent RNAP-promoter complex (RPO). Despite extensive study, the mechanism of promoter unwinding has remained unclear, in part due to the transient nature of intermediates on path to RPo. Here, using single-molecule unwinding-induced fluorescence enhancement to monitor promoter unwinding, and single-molecule fluorescence resonance energy transfer to monitor RNAP...