Duyệt theo

Hồ sơ tác giả

Tìm kiếm theo: Chủ đề mRNA

Duyệt theo: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
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Hiển thị kết quả tìm kiếm từ 1 đến 8 trong 8 kết quả phù hợp
  • OER000002664.pdf.jpg
  • Journal article

  • APC couples neuronal mRNAs to multiple kinesins, EB1 and shrinking microtubule ends for bidirectional mRNA motility

    • Tác giả : Baumann, Sebastian J. (2023)

  • Understanding where in the cytoplasm mRNAs are translated is increasingly recognised as being as important as knowing the timing and level of protein expression. mRNAs are localised via active motor-driven transport along microtubules (MTs) but the underlying essential factors and dynamic interactions are largely unknown. Using biochemical in vitro reconstitutions with purified mammalian proteins, multi-colour TIRF-microscopy (TIRF-M), and interaction kinetics measurements, we s...
  • OER000000809.pdf.jpg
  • Journal article

  • Decoding mRNA translatability and stability from 5’UTR

    • Tác giả : Jia, Longfei; Mao, Yuanhui; Ji, Quanquan (2020)

  • Precise control of protein synthesis by engineering sequence elements in 5’ untranslated region (5’UTR) remains a fundamental challenge. To accelerate our understanding of cis-regulatory code embedded in 5’UTR, we devised massively parallel reporter assays from a synthetic mRNA library composed of over one million 5’UTR variants. A completely randomized 10-nucleotide sequence preceding an upstream open reading frame (uORF) and downstream GFP leads to a broad range...
  • OER000000400.pdf.jpg
  • Journal article

  • Dynamic regulation of translation quality control associated with ribosome stalling

    • Tác giả : Goldman, Daniel H; Livingston, Nathan M; Movsik, Jonathan (2020)

  • Translation of problematic mRNA sequences induces ribosome stalling. Collided ribosomes at the stall site are recognized by cellular quality control machinery, resulting in dissociation of the ribosome from the mRNA and subsequent degradation of the nascent polypeptide and in some organisms, decay of the mRNA. However, the timing and regulation of these processes are unclear. We developed a SunTag-based reporter to monitor translation in real time on sin...
  • OER000002695.pdf.jpg
  • Journal article

  • Methylated guanosine and uridine modifications in S. cerevisiae mRNAs modulate translation elongation

    • Tác giả : Jones, Joshua D. (2023)

  • Chemical modifications to protein encoding messenger RNA (mRNA) can modulate their localization, translation and stability within cells. Over 15 different types of mRNA modifications have been identified by sequencing and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) technologies. While LC-MS/MS is arguably the most essential tool available for studying analogous protein post-translational modifications, the high-throughput discovery and quantitative characteri...
  • OER000002694.pdf.jpg
  • Journal article

  • N1-Methylpseudouridine and pseudouridine modifications modulate mRNA decoding during translation

    • Tác giả : Monroe, Jeremy G. (2023)

  • rapid and accurate protein production. The inclusion of chemically modified bases into mRNAs has the potential to alter the strength and pattern of hydrogen bonding between mRNAs and aminoacyl-tRNAs to alter protein synthesis. We investigated how the N1-methylpseudouridine (m1Y) modification, commonly incorporated into therapeutic and vaccine mRNA sequences, influences the ability of codons to react with cognate and near-cognate tRNAs and release factors. We find ...
  • OER000002658.pdf.jpg
  • Journal article

  • Structural basis for reduced ribosomal A-site fidelity in response to P-site codon-anticodon mismatches

    • Tác giả : Nguyen, Ha An (2023)

  • Rapid and accurate translation is essential in all organisms to produce properly folded and functional proteins. mRNA codons that define the protein coding sequences are decoded by tRNAs on the ribosome in the aminoacyl (A) binding site. The mRNA codon and the tRNA anticodon interaction is extensively monitored by the ribosome to ensure accuracy in tRNA selection. While other polymerases that synthesize DNA and RNA can correct for...
  • OER000004043.pdf.jpg
  • Journal article

  • The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5’ cap modulates protein expression in living cells

    • Tác giả : Sikorski, Pawel J; Warminski, Marcin; Kubacka, Dorota (2019)

  • 7-Methylguanosine 5’-cap on mRNA is necessary for efficient protein expression in vitro and in vivo. Recent studies revealed structural diversity of endogenous mRNA caps, which carry different 5’-terminal nucleotides and additional methylations (2’-O-methylation and m6A). Currently available 5’-capping methods do not address this diversity. We report trinucleotide 5’-cap analogs (m7GpppN(m)pG), which are utilized by RNA polymerase T7 to initiate transcription from templates carr...
  • OER000000670.pdf.jpg
  • Journal article

  • The kinetic landscape of an RNA binding protein in cells

    • Tác giả : Sharma, Deepak; Zagore, Leah L; Brister, Matthew M (2020)

  • Gene expression in higher eukaryotic cells orchestrates interactions between thousands of RNA binding proteins (RBPs) and tens of thousands of RNAs 1. The kinetics by which RBPs bind to and dissociate from their RNA sites are critical for the coordination of cellular RNA-protein interactions 2. However, these kinetics were experimentally inaccessible in cells. Here we show that time-resolved RNA-protein crosslinking with a pulsed femtosecond UV laser, followe...