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Browsing by Subject methyl hóa

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Showing results 1 to 5 of 5

Journal article

A CRISPR/Cas12a-assisted platform for identification and quantification of single CpG methylation sites

Authors : Dongen, J.E. van (2021)

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/associated nuclease (Cas) systems have repeatedly shown to have excellent performance in nucleotide sensing applications1– 8 5 9 . High specificity and selectivity of Cas effector proteins is determined by the CRISPR RNA’s (crRNA’s) interchangeable spacer sequence, as well as position and number of mismatches between target sequence and the crRNA sequence1 11 . Some diseases are characterized ...

Journal article

Methylated guanosine and uridine modifications in S. cerevisiae mRNAs modulate translation elongation

Authors : Jones, Joshua D. (2023)

Chemical modifications to protein encoding messenger RNA (mRNA) can modulate their localization, translation and stability within cells. Over 15 different types of mRNA modifications have been identified by sequencing and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) technologies. While LC-MS/MS is arguably the most essential tool available for studying analogous protein post-translational modifications, the high-throughput discovery and quantitative characteri...

Journal article

Methylation in NDUFA13 gene promoter disrupts communication between collaborative transcription factors – potential mechanism for onset of breast cancer

Authors : Johanna, Hörberg (2023)

Selective DNA binding by transcription factors (TFs) is crucial for the correct regulation of DNA transcription. In healthy cells, promoters of active genes are hypomethylated. A single CpG methylation within a TF response element may change the binding preferences of the protein thus causing the dysregulation of transcription programs. Here we investigate a molecular mechanism driving the downregulation of NDUFA13 gene, due to hypermethylation, ...

Journal article

Studies in CRISPR-generated Mediterranean G6PD variant rats reveal G6PD orchestrates genome-wide DNA methylation and gene expression in vascular wall

Authors : Signoretti, Christina (2023)

Recent advances have revealed the importance of epigenetic modifications to gene regulation and transcriptional activity. DNA methylation, a determinant of genetic imprinting and de novo silencing of genes genome-wide, is known to be controlled by DNA methyltransferases (DNMT) and demethylases (TET) under disease conditions. However, the mechanism(s)/factor(s) influencing the expression and activity of DNMTs and TETs, and thus DNA methylation, in he...

Journal article

The Burden Borne by Protein Methyltransferases: Rates and Equilibria of Nonenzymatic Methylation of Amino Acid Side-Chains by SAM in Water

Authors : Lewis Jr, Charles A. (2021)

SAM is a powerful methylating agent, with a methyl group transfer potential matching the phosphoryl group transfer potential of ATP. SAM-dependent N-methyltransferases have evolved to catalyze the modification of specific lysine residues in histones and transcription factors, in addition to generating epinephrine, N-methylnicotinamide, and a quaternary amine (betaine) that is used to maintain osmotic pressure in plants and halophilic bacteria. To assess&...