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  • Authors : Chimari, Jiko (2023)

  • Mammalian F-ATP synthase is central to mitochondrial bioenergetics and is present in the inner mitochondrial membrane in a dynamic oligomeric state of higher oligomers, tetramers, dimers and monomers. In vitro investigations of mammalian F-ATP synthase are often limited by the ability to purify the oligomeric forms present in vivo at sufficient quantity, stability and purity. We developed a purification approach for the isolation of bovine ...