Browse

Author Profile

Browsing by Subject nấm men

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:  
Showing results 1 to 6 of 6
  • OER000002654.pdf.jpg
  • Journal article

  • Biochemical and Genetic Evidence Supports Fyv6 as a Second-Step Splicing Factor in Saccharomyces cerevisiae

    • Authors : Lipinski, Karli A. (2023)

  • Precursor mRNA (pre-mRNA) splicing is an essential process for gene expression in 15 eukaryotes catalyzed by the spliceosome in two transesterification steps. The spliceosome is a 16 large, highly dynamic complex composed of 5 small nuclear RNAs and dozens of proteins, some 17 of which are needed throughout the splicing reaction while others only act during specific stages. 18 The human protein FAM192A was recently proposed to be&...
  • OER000002501.pdf.jpg
  • Journal article

  • Branched chain amino acid synthesis is coupled to TOR activation early in the cell cycle in yeast

    • Authors : Blank, Heidi M.  (2023)

  • How cells coordinate their metabolism with division determines the rate of cell proliferation. Dynamic patterns of metabolite synthesis during the cell cycle are unexplored. We report the first isotope tracing analysis in synchronous, growing budding yeast cells. Synthesis of leucine, a branched-chain amino acid (BCAA), increased through the G1 phase of the cell cycle, peaking later during DNA replication. Cells lacking Bat1, a mitochondrial&#x...
  • OER000002534.pdf.jpg
  • Journal article

  • Purification of Human β- and γ-actin from Budding Yeast

    • Authors : Haarer, Brian K.  (2023)

  • Biochemical studies of human actin and its binding partners rely heavily on abundant and easily purified α-actin from skeletal muscle. Therefore, muscle actin has been used to evaluate and determine the activities of most actin regulatory proteins and there is an underlying concern that these proteins perform differently with actin present in non-muscle cells. To provide easily accessible and relatively abundant sources of human β-&#...
  • OER000002373.pdf.jpg
  • Journal Article

  • The mechanism of Atg15-mediated membrane disruption in autophagy

    • Authors : Kagohashi, Yoko (2023)

  • Autophagy is a lysosomal/vacuolar delivery system that isolates and degrades cytoplasmic material. Following delivery by autophagosomes, cytoplasmic components are released into the vacuole within an autophagic body (AB), which is a single-membrane structure derived from the inner membrane of the autophagosome. This membrane must be disrupted for degradation of the cytoplasmic cargo to occur. The vacuolar proteases Pep4 and Prb1, as well as the&...
  • OER000002548.pdf.jpg
  • Journal article

  • Utilizing a nanobody recruitment approach for assessing serine palmitoyltransferase activity in ER sub-compartments of yeast

    • Authors : Esch, Bianca M. (2023)

  • Sphingolipids (SP) are one of the three major lipid classes in eukaryotic cells and serve as38 structural components of the plasma membrane. The rate-limiting step in SP biosynthesis is39 catalyzed by serine palmitoyltransferase (SPT). In yeast, SPT consists of two catalytic subunits40 (Lcb1 and Lcb2), a regulatory subunit (Tsc3), negative regulators (Orm1 and Orm2), and the41 phosphatidylinositol-4-phosphate (PI4P) phosphatase Sac1, collectively known as...
  • OER000002315.pdf.jpg
  • Journal Article

  • Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast

    • Authors : Courtin, Baptiste (2023)

  • Gene expression is a crucial and well-orchestrated interplay between transcription, translation, and mRNAs and proteins decay. In eukaryotes, cytoplasmic mRNA decay is intimately linked to translation and mediated for a large part by the 5’- 3’ mRNA degradation pathway. After mRNA decapping by the DCP complex, with the assistance of its co-factors, the mRNA is degraded by the exonuclease Xrn1 (Parker 2012; Fromont-Racine and Saveanu 2014;&#...