Journal articleAuthors : Schessner, Julia P. (2023)
The Dynamic Organellar Maps (DOMs) approach combines cell fractionation and shotgun-proteomics for global
profiling analysis of protein subcellular localization. Here, we have drastically enhanced the performance of
DOMs through data-independent acquisition (DIA) mass spectrometry (MS). DIA-DOMs achieve twice the depth
of our previous workflow in the same MS runtime, and substantially improve profiling precision and
reproducibility. We leveraged this gain to e... 
