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Showing results 1 to 4 of 4

Journal article

A metal ion-dependent mechanism of RAD51 nucleoprotein filament disassembly

Authors : Appleby, Robert (2023)

The RAD51 ATPase polymerises on single-stranded DNA to form nucleoprotein filaments (NPFs) that are critical intermediates in the DNA strand-exchange reactions of Homologous Recombination (HR). ATP binding is important to maintain the NPF in a competent conformation for strand pairing and exchange. Once strand exchange is completed, ATP hydrolysis licenses the filament for disassembly. Here we show using high-resolution cryoEM that the ATP-binding&#...

Journal Article

In-Depth Characterization of Apoptosis N-terminome Reveals a Link Between Caspase-3 Cleavage and Post-Translational N-terminal Acetylation

Authors : Hanna, Rawad (2023)

The N-termini of proteins contain information about their biochemical properties and functions. These Ntermini can be processed by proteases and can undergo other co- or post-translational modifications. We have developed LATE (LysN Amino Terminal Enrichment), a method that uses selective chemical derivatization of α-amines to isolate the N-terminal peptides, in order to improve N-terminome identification in conjunction with other enrichment strategies. We...

Journal article

Mer3 helicase protects early crossover intermediates from STR complex disassembly during meiosis

Authors : Altmannova, Veronika (2023)

During meiosis I it is necessary that homologous chromosomes are linked to one another so that they can be faithfully separated. S. cerevisiae Mer3 (HFM1 in mammals) is a SF2 helicase and member of the ZMM group of proteins, that facilitates the formation of class I crossovers during meiosis. Here we describe the structural organisation of Mer3 and, using AlphaFold modelling and XL-MS, we further characterise the previ...

Journal article

Structural basis of ubiquitin-independent PP1 complex disassembly by p97

Authors : Boom, Johannes van den (2023)

The AAA+ ATPase p97 (also called VCP, or Cdc48 in yeast) unfolds proteins and disassembles protein complexes in a myriad of cellular processes, but how a substrate complex needs to be loaded onto p97 by a dedicated substrate adapter and then disassembled by p97 has not been structurally visualized so far. Here we present cryo-EM structures of p97 in the process of disassembling a protein phosphatase-1 (PP1) complex by &#...